To understand the effect of firewood ash on vital organs of locally available earthworm Lampito mauritti was selected.The biochemical studies like protein, Lipid and Glycogen content in fore region, middle region and hind region of both control and wood ash treated experimental group of earthworm have been carried out.LC50 value was calculated before the experiment by the following method of Saptami Moitra and Verma (1997).The earthworm are divided into two groups, one is control (N=50) group another one is called experimental (N=50) group. The control group of earthworms was cultured in native soil with cow dung. The experimental group of earthworm cultured in 1/10 of LC50 value (50g/kg of soil) of firewood ash with native soil and cow dung. The experimental period was one month (30 days).After 30 days of experiment earthworms from each group were collected and dissected out for the analysis of biochemical and histological studies. After 30 days of experiment the concentration of protein in the control group earthworm showed 11.4±0.22 µg/mg in fore region, 32.7±0.16 µg/mg in mid region and 12.6±0.10 µg/mg in hind region. But in the case of experimental group the earthworm showed highly reduced level of protein that is 6.4±0.10 µg/mg in fore region, 15.5±0.12 µg/mg in middle region and 11.3±0.11 µg/mg in hind region. After 30 days of experiment the Lipid content in both group showed variation. The glycogen content after 30 days of experiment. The control earthworm showed 24.9±0.22 µg/mg in fore region, 19.7±0.10 µg/mg in middle region and high amount of glycogen in hind region (29.5±0.28 µg/mg) were noticed. The experimental group of earthworm showed highly decreased level of glycogen content when compared to control group. This may be due to electricity production waste that is firewood ash contain the large amount of chemical substance called potassium oxide this may enter in to the body via food and alter the biochemical parameters.