Objective: Dengue is the most important arthropod-borne viral diseases in humans with a large worldwide burden. There are an estimated 50 million infections per year occurring across approximately 100 countries in tropical and sub-tropical regions in the world and potential for further spread. Methods: Serological and RT-PCR Results: RT-PCR shown high sensitive with diagnosis of different serotypes as compared to serological tests. Conclusion: Highly Sensitive and specific diagnosis at species level is prime important for treatment, epidemiological study and control of such infection along with tretment.