Based on last due decades, job satisfaction assumed as one of the principle organizational factors that has considerable role amongstacademic staff at university; moreover, concentrating on this imperative factor and discoveryoperative items that effect on the level of job satisfaction is very critical. The main purpose of current research is to survey the relationships between gender, marital status, level of education, reward, and status of designationwith job satisfaction of academic staff at Malaysian universities. The Job Descriptive Index (JDI) was applied to measures job satisfaction amongst academic staff. The data of presentstudy taken from 440 academic staff were occupied in Malaysian universities. The findings illustrated there were significant relationships between level of education, reward, and status of designation with job satisfaction, while there were not meaningful association between gender and marital status with job satisfaction; that analyzed by t-test.