Environmental contamination by toxic heavy metals is causing a serious problem worldwide due to their incremented accumulation in food chain and continued persistence ecosystem. Sixty-five isolates of actinomycetes were isolated from different regions in Egypt. The eleven isolates which were removed any studied heavy metals, cu2+, pb2+ and cd2+. singly above 70% was selected to remove metals from ternary mixture for studied metals ions. The highest removing percentage was recorded by kw27 isolate which was removed 70.8 %, 76.3 %, and 61.7 % from Cu2+, Pb2+ and Cd2+ respectively. The 16S rRNA analyses and phylogenetic data of kw27 concluded that Kw27 was member of Nocardia genus and kw27 was deposited in the Gen Bank Database under accession number MK014899. The results of the dead biomass of Nocardia takedensis Kw27 for Cu2+, Pb2+, and Cd2+ under the optimized conditions pH-8 at 450C for 3hours with 0.3% biosorbent dosage was found to be as follows: Pb2+, 87.2 %. > Cu2+, 79.7 %. >Cd2+, 71.3 %). All studied heavy metals were removed from aqueous solutions when the sorbent dosage increased to 0.6% or when contact time increased from 5 to 6 hours by dead biomass of Kw27 isolate. This work conformed the potential use of Nocardia takedensis Kw27 as an inexpensive and efficient technology for removal of cu2+, pb2+ and cd2 from aqueous solutions.
