Chrysanthemum is commonly known as Autumn Queen. It belongs to the family Compositeae (Asteraceae). It is highly attractive and charming short day plant, which behaves both as an annual as well as perennial flowering herb. Regeneration of Chrysanthemum plantlets through in vitro culture was obtained by using nodal segment explants. These explants were treated with different concentrations of auxins (IAA, IBA) and cytokinins (BAP) alone and in combinations. Nodal segments of chrysanthemum when placed on MS medium supplemented with different concentrations of BAP with IAA. The highest results were observed on MS medium containing 1.0 mg/l BAP + 0.1 mg/l IAA, shown 90% shoot initiation and 5.5 ± 0.51 average length of shoot per explant. Nodal segment of chrysanthemum were cultured in MS media with different concentration of BAP. The highest results were observed on MS medium containing 1.0 mg/l BAP, shown 93% shoot proliferation, 5.7 ± 0.22 average lengths of shoot per explant and 4.4±0.88 nodes per explant. The regenerated shoot lets were rooted on MS medium and ½ MS medium with different concentration of IBA. The highest results were observed on ½ MS medium containing 0.2 mg/l IBA shown 90% micro cuttings rooted. 9 ± 0.19 average length of roots was observed per explant and 11.8 ± 0.75 number per explant of chrysanthemum.