Culture of leaves and immature fruits of maerua crassifolia in vitro

Leaves and immature fruits of M. crassifolia were cultured on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins and cytokinins. When the leaves were inoculated on MS +Indolebutyric acid (IBA) 1 mg/l, a small green branches were directly formed from basal part as well as radicles covered with root hairs after two weeks, the branches were increased in growth and reached 0.3, 1and 1.3 cm in length after 14, 30,45 days respectively. Those branches were sub cultured on MS+ Benzyl adenine (BA) (2mg|l), no any further responses were observed, but their color was changed to brown after two weeks. In the case of using immature fruits as explants on MS+2,4-Dichlorophenoxy acetic acid 2,4-D (2mg/l)+kinetin (0.5mg/l), friable white calluses were obtained after 7 days, their growth were increased after 12 weeks. When the callus subculture on MS+2,4-D (2mg/l)+kin (1.5mg/l) and on MS+ Naphthalene acetic acid NAA(1.5 mg/l) + kin (3.5mg/l), no any responses were noticed after 4 weeks.