Prophylactic Role Of Carissa Carandas Against Dmba Induced Skin Carcinogenesis In Swiss Albino Mice

The aim of the present study is to elucidate the protective effect of Carissa Carandas fruit extract (CCE) on skin carcinogenesis induced by a single topical application of 7, 12-dimethylbenz (a) anthracene (100 μg/100 μl of acetone) and after 2 weeks repeated application of croton oil (1% in acetone/three times a week) till the end of the experiment (i.e. 16 weeks). Oral administration of CCE at a dose of 50 mg/kg b.wt./day during Peri initiational stage (i.e. 7 days before & 7 days after DMBA application followed by croton oil till the end of experiment; Group IV) and Post initiational stage (from the day of croton oil treatment till the end of the experiment; Group V) reduced tumor burden, tumor yield, cumulative number of papilloma as compared to DMBA/croton oil treated group (Group III) . Group IV and V showed a significant reduction in lipid peroxidation along with an elevation in antioxidant enzymes (superoxide dismutase and catalase), non-enzymatic antioxidant (reduced glutathione) and total proteins levels in the liver and skin of mice when compared to carcinogen treated control (Group III).