An analytically cross-sectional study was conducted to evaluate the presence of work related musculoskeletal disorders among call-centre employees. Two hundred and twenty seven employees selected at random formed the sample for the investigation. A self—reporting questionnaire was used to collect data on the practice of physical exercise, the presence of pain during or soon after the work schedule and the adoption of preventive measures related to clinical activities. Results were analysed. The x2 test was used to identify associations between variables. The presence of pain during or after clinical wok was reported by 76.2 per cent respondents. Statistically significant differences were found between gender and the occurrence of pain. Pain was present during work (C=0.006) and imposed limitations on work routine (P=0.011). The high percentage of pain reported by call center employees suggest reviewing work conditions in call centers in order to reduce employees’ exposure to risk factors for developing musculoskeletal disorders.